p irf3 (Cell Signaling Technology Inc)
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96
Structured Review
Cell Signaling Technology Inc
p irf3
P Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 632 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+irf3+ser396/Phospho-IRF-3+(Ser396)+Rabbit+mAb/pmc12813888-103-34-36
Average 96 stars, based on 632 article reviews
P Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 632 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+irf3+ser396/Phospho-IRF-3+(Ser396)+Rabbit+mAb/pmc12813888-103-34-36
Average 96 stars, based on 632 article reviews
p irf3 - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "On-demand mild photothermal cascade platform reprogramming mitochondrial immunity for tendon rejuvenation"
Article Title: On-demand mild photothermal cascade platform reprogramming mitochondrial immunity for tendon rejuvenation
Journal: Bioactive Materials
doi: 10.1016/j.bioactmat.2026.01.004
Figure Legend Snippet: Schematic illustration of the ROS-responsive on-demand mild photothermal cascade platform for tendon rejuvenation in Achilles tendinopathy. The platform strategically modulates the mitochondrial-cGAS-STING-IRF3/NF-κB signaling axis and induces heat shock protein 70 (HSP70) expression to attenuate oxidative stress and cellular senescence within tendon stem/progenitor cells (TSPCs). Consequently, it promotes tenogenic differentiation while abrogating aberrant osteogenic/chondrogenic lineage commitment. This cascade effect ultimately mitigates heterotopic ossification, enhances structural tendon regeneration, restores biomechanical function, and alleviates pain. Nanoparticle nomenclature: LA-NPs and TPA-TCNQ-NPs denote nanoparticles encapsulating LA or TPA-TCNQ individually; LT-NPs refers to the composite formulation consisting of a mixture of LA-NPs and TPA-TCNQ-NPs; and LT-NPs-NIR represents the LT-NPs mixture following 808 nm near-infrared (NIR) irradiation to activate the photothermal response and controlled payload release.
Techniques Used: Expressing, Formulation, Irradiation
Figure Legend Snippet: LT-NPs-NIR regulate the mtDNA-STING-IRF3/NF-κB pathway in macrophages. (A) Principal component analysis (PCA) of transcriptomic data from RAW 264.7 cells under different treatments. (B) Volcano plots comparing H 2 O 2 vs. Control (left) and LT-NPs-NIR vs. H 2 O 2 (right). (C) Heatmap of differentially expressed genes (DEGs) with hierarchical clustering. (D, E) Gene Ontology (GO), KEGG, and Gene Set Enrichment Analysis (GSEA) for H 2 O 2 vs. Control (D) and LT-NPs-NIR vs. H 2 O 2 (E). (F) Multi-SIM imaging showing mtDNA (magenta) and the mitochondrial outer membrane protein TOMM20 (green), with quantification of their colocalization. (G) Western blot analysis of key proteins in the cGAS-STING-NF-κB axis. Scale bar: 5 μm (F). Significance: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
Techniques Used: Control, Imaging, Membrane, Western Blot
Figure Legend Snippet: LT-NPs-NIR attenuate oxidative stress, preserve mitochondrial integrity, and suppress senescence in TSPCs by inhibiting the mtDNA-STING-NF-κB axis. (A) Schematic of the experimental design. (B) TSPC proliferation assessed by CCK-8 assay. (C, E) Immunofluorescence staining and quantification of HSP70 (n = 3). (D, F) Mitochondrial membrane potential (ΔΨm) visualized by JC-1 staining (red: high potential; green: low potential) and quantification. (G) Multi-SIM of mtDNA (magenta) and TOMM20 (green) with colocalization analysis. (H) Western blot of cGAS-STING-IRF3-NF-κB pathway proteins. (I, K) Colony-forming unit fibroblast (CFU-F) assay and quantification of self-renewal capacity (n = 3). (J) Senescence-associated β-galactosidase (SA-β-gal) activity. (L, M) Apoptosis analysis by Annexin V/PI flow cytometry and quantification (n = 3). Scale bars: 100 μm (C); 5 μm (D, G); 200 μm (J). Significance: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
Techniques Used: CCK-8 Assay, Immunofluorescence, Staining, Membrane, Western Blot, Activity Assay, Flow Cytometry
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